Webbing was a web fluid invented by Peter Parker meant to simulate a spider's web.

When Spider-Man taps into his web shooter trigger, the web fluid is shot from a small nozzle. When the web fluid comes in contact with the air, it solidifies into a rope-like material that Spider-Man uses to web-swing. If Spider-Man taps the trigger hard enough, the nozzle widens its opening, making the web coming out of the web shooter wider. The webbing produced by these cartridges is ultra-strong but dissolves in about one hour’s time.

Spider-Man has improved his webbing formula over the years as he gained access to better labs and chemicals, and he has also developed variations on his web fluid formula for use against specific villains such as when he fights Venom he has an extremely strong and sticky webbing, insulated webbing against Electro, acidic webbing to melt through Rhino's tough hide and flame-retardant webbing to fight fires or fire elemental enemies.

When Ben Riley took over the mantle as Spider-Man he created a formula that wrapped opponents on impact when shot but had the same tensile strength as normal web fluid. It was called Impact Webbing.